| Performance |
WAVE |
Mixing tank |
Spinner Flask/Shake Flask |
| Comparison |
| Mixed mode |
Wavy |
Immersion stirring |
Swinging vortex type |
| Shear force |
Extremely low |
High |
Low |
| Oxygen transfer area |
High |
Middle |
Low |
| Oxygen transmission mode |
Surface wave type oxygen transfer |
Bubbling oxygen transfer |
Surface oxygen transfer |
| Oxygen transmission effect |
High |
Middle |
Low |
| Dissolved oxygen level |
High |
Middle |
Low |
| Gas flow meter |
High precision mass flowmeter |
Cheap rotameter,low accuracy |
NA |
| Cell density(cell/mL) |
High density,generally >106-7 T CELL perfusion 1~2x107
Insect cell perfusion >108 |
106-7 |
Low density,generally1055-6 |
| Cross contamination risk |
NA |
High |
High |
| Infection probability |
Low |
Middle |
High |
| Cluture parameter control |
Automatic feedback regulation pH,DO,Temp, CO₂% |
Automatic feedback regulation pH,
DO,Temp, CO₂% |
NA |
| Weighing perfusion |
Integrated weighing and automatic filling |
NA |
NA |
| Working volume |
Flexible,10-100% |
Inflexible,50-100% |
Inflexible，10-30% |
| Foam |
Positive pressure,not easy to produce foam |
Large amount of foam will be
generated,and a defoamer is required |
Less foam |
| Operation |
Easy |
A lot of experience needed |
Difficult to scale up applicable |
| Microcarrier technology |
applicable |
applicable |
NA |
| Micro carrier ball |
It can be performed directly in the Wave without a digestion reactor |
The operation is complicated and needs
to be transferred to the digestion reactor. |
/ |
| Training environment |
Ordinary |
Clean room |
Clean room |
| mobility |
Yes |
Fixed |
Yes |
| Comparison of process scale-up production |
| Scale up mode |
00mL-100L requires only one WAVE,
or multiple 100L WAVES for parallel scale up |
Three to four seed tanks are required
for step by step to above 1000L. |
Large amount of spinner
flasks/shake flasks |
| Scale up |
Easy,fine tune parameters |
High risk,need to explore again |
Difficult to scale up |
| Equipment cost |
Low |
High |
Low |
| Computer real-time monitoring |
Yes |
Yes |
No |
Multi variety R&D and
production platform |
Sterile closed piped platform
enables quick change of cell
bags for easy authentication. |
Difficult to clean and certificate,
and cross contamination among varieties |
Difficulties in
managing certification |
| Equipment maintenance cost |
Low |
High |
Low |
| Cost of new workshop |
1(Class 10000 trolley room) |
10(Class 100 medium workshop) |
4(Class 10000 workshop) |
Expansion and utilization
of existing workshops |
No reconstruction required |
Redesign and build |
Extension |
| Workshop construction |
Short cycle and low cost |
Long cycle and high cost |
Long cycle and high cost |
| Production cycle |
Short |
Long |
Short |
| Fixed stainless steel pipe connection |
No |
Yes |
No |
| Production flexibility |
High |
Low |
Low |
| Auxiliary production cycle |
Short |
Long |
Long |
| Plant utilization rate |
High |
Low |
Low |
| SIP steam |
No |
Much |
No |
| WFI dosage |
Very little |
Much |
Middle |
| Cleaning and maintenance cost |
Extremely low |
Very high |
High |
| Consumables |
Cell bags(Cell bags of the same cells can be reused) |
Mixing energy consumption,
pipe sealing,valve gasket,
cleaning solution,WFl,SIP steam |
Cell flasks,cleaning
solutions,WFI and
extensive manual work |
